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Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb

Balakrishnan Arunachalam, Kokilavani Chinnaraju.

Abstract
Aim: The present study involves in the investigation of various extracts of Cucumis trigonus Roxb. for its phytochemical constituents and also the cytotoxic potential of the Cucumis trigonus Roxb. ethanolic fruit extract.
Methods: The phytochemical screening with methanol, ethanol, petroleum ether, chloroform and aqueous extracts of Cucumis trigonus Roxb. was done by modern method of Peach and Tracey (1955) Cytotoxicity is estimated by staining technique using Trypan blue after the addition of drug, the dead cells are stained blue with Trypan blue. Confirmation studies were done by additional metabolic intervention experiments like (3-(4, 5-dimethyl thiazol-2yl)-2, 5-diphenyl tetrazolium bromide) MTT assays.
Results: Qualitative phytochemical analysis of the plant extracts were done and it was found that the presence of phytochemicals were maximum in the ethanolic extract when compared to other extracts. So the ethanolic extract of C. trigonus fruit was used for further investigations. In the cytotoxic study the ethanolic fruit extract concentration at 1.25 mg / ml was found to be the effective dose because at this concentration, it exhibited 50% cytotoxicity against Hep2 cells.
Conclusion: These results conclude that the ethanolic fruit extract of Cucumis trigonus possess a good phytochemical strength and also an effective cytotoxic potential.

Key words: Cucumis trigonus, phytochemical, Trypan blue, Cytotoxicity


 
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How to Cite this Article
Pubmed Style

Arunachalam B, Chinnaraju K. Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb. J Intercult Ethnopharmacol. 2012; 1(1): 13-18. doi:10.5455/jice.20120117121046



Web Style

Arunachalam B, Chinnaraju K. Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb. www.scopemed.org/?mno=13783 [Access: November 18, 2017]. doi:10.5455/jice.20120117121046



AMA (American Medical Association) Style

Arunachalam B, Chinnaraju K. Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb. J Intercult Ethnopharmacol. 2012; 1(1): 13-18. doi:10.5455/jice.20120117121046



Vancouver/ICMJE Style

Arunachalam B, Chinnaraju K. Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb. J Intercult Ethnopharmacol. (2012), [cited November 18, 2017]; 1(1): 13-18. doi:10.5455/jice.20120117121046



Harvard Style

Arunachalam, B. & Chinnaraju, K. (2012) Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb. J Intercult Ethnopharmacol, 1 (1), 13-18. doi:10.5455/jice.20120117121046



Turabian Style

Arunachalam, Balakrishnan, and Kokilavani Chinnaraju. 2012. Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb. Journal of Intercultural Ethnopharmacology, 1 (1), 13-18. doi:10.5455/jice.20120117121046



Chicago Style

Arunachalam, Balakrishnan, and Kokilavani Chinnaraju. "Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb." Journal of Intercultural Ethnopharmacology 1 (2012), 13-18. doi:10.5455/jice.20120117121046



MLA (The Modern Language Association) Style

Arunachalam, Balakrishnan, and Kokilavani Chinnaraju. "Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb." Journal of Intercultural Ethnopharmacology 1.1 (2012), 13-18. Print. doi:10.5455/jice.20120117121046



APA (American Psychological Association) Style

Arunachalam, B. & Chinnaraju, K. (2012) Preliminary phytochemical analysis and cytotoxic potential of Cucumis trigonus Roxb. Journal of Intercultural Ethnopharmacology, 1 (1), 13-18. doi:10.5455/jice.20120117121046





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