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Extracts of Cordia gilletii De Wild (Boraginaceae) Quench the Quorum Sensing of Pseudomonas aeruginosa PAO1

Philippe N. Okusa, Tsiry Rasamiravaka, Olivier Vandeputte, Caroline Stévigny, Mondher El Jaziri, Pierre Duez.

Cited by (2)

Abstract
Aim: The fight against infectious diseases and antimicrobial resistances needs the exploration of new active compounds with new proprieties such as disrupting quorum sensing (QS) mechanisms, which is a cell-to-cell communication that regulates bacterial virulence factors. In this work, leaves and root barks extracts of a Congolese medicinal plant, Cordia gilletii, were investigated for their effect on the production of Pseudomonas aeruginosa major virulence factors regulated by QS. Material and methods: The effect of Cordia gilletii extracts on virulence factors of Pseudomonas aeruginosa PAO1 was studied by the evaluation of the production of pyocyanine, elastase and biofilm; and by the measurement of the expression of QS-related genes. Results: The dichloromethane extract from root barks was found to quench the production of pyocyanin, a QS-dependent virulence factor in P. aeruginosa PAO1. Moreover, this extract specifically inhibits the expression of several QS-regulated genes (i.e. lasB, rhlA, lasI, lasR, rhlI and rhlR) and reduces biofilm formation by PAO1. Conclusion: This study contributes to explain the efficacy of Cordia gilletii in the traditional treatment of infectious diseases caused by P. aeruginosa.

Key words: Cordia gilletii, antimicrobial, quorum-sensing


 
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How to Cite this Article
Pubmed Style

Okusa PN, Rasamiravaka T, Vandeputte O, Stévigny C, Jaziri ME, Duez P. Extracts of Cordia gilletii De Wild (Boraginaceae) Quench the Quorum Sensing of Pseudomonas aeruginosa PAO1. J Intercult Ethnopharmacol. 2014; 3(4): 138-143. doi:10.5455/jice.20140710031312



Web Style

Okusa PN, Rasamiravaka T, Vandeputte O, Stévigny C, Jaziri ME, Duez P. Extracts of Cordia gilletii De Wild (Boraginaceae) Quench the Quorum Sensing of Pseudomonas aeruginosa PAO1. www.scopemed.org/?mno=161340 [Access: November 18, 2017]. doi:10.5455/jice.20140710031312



AMA (American Medical Association) Style

Okusa PN, Rasamiravaka T, Vandeputte O, Stévigny C, Jaziri ME, Duez P. Extracts of Cordia gilletii De Wild (Boraginaceae) Quench the Quorum Sensing of Pseudomonas aeruginosa PAO1. J Intercult Ethnopharmacol. 2014; 3(4): 138-143. doi:10.5455/jice.20140710031312



Vancouver/ICMJE Style

Okusa PN, Rasamiravaka T, Vandeputte O, Stévigny C, Jaziri ME, Duez P. Extracts of Cordia gilletii De Wild (Boraginaceae) Quench the Quorum Sensing of Pseudomonas aeruginosa PAO1. J Intercult Ethnopharmacol. (2014), [cited November 18, 2017]; 3(4): 138-143. doi:10.5455/jice.20140710031312



Harvard Style

Okusa, P. N., Rasamiravaka, T., Vandeputte, O., Stévigny, C., Jaziri, M. E. & Duez, P. (2014) Extracts of Cordia gilletii De Wild (Boraginaceae) Quench the Quorum Sensing of Pseudomonas aeruginosa PAO1. J Intercult Ethnopharmacol, 3 (4), 138-143. doi:10.5455/jice.20140710031312



Turabian Style

Okusa, Philippe N., Tsiry Rasamiravaka, Olivier Vandeputte, Caroline Stévigny, Mondher El Jaziri, and Pierre Duez. 2014. Extracts of Cordia gilletii De Wild (Boraginaceae) Quench the Quorum Sensing of Pseudomonas aeruginosa PAO1. Journal of Intercultural Ethnopharmacology, 3 (4), 138-143. doi:10.5455/jice.20140710031312



Chicago Style

Okusa, Philippe N., Tsiry Rasamiravaka, Olivier Vandeputte, Caroline Stévigny, Mondher El Jaziri, and Pierre Duez. "Extracts of Cordia gilletii De Wild (Boraginaceae) Quench the Quorum Sensing of Pseudomonas aeruginosa PAO1." Journal of Intercultural Ethnopharmacology 3 (2014), 138-143. doi:10.5455/jice.20140710031312



MLA (The Modern Language Association) Style

Okusa, Philippe N., Tsiry Rasamiravaka, Olivier Vandeputte, Caroline Stévigny, Mondher El Jaziri, and Pierre Duez. "Extracts of Cordia gilletii De Wild (Boraginaceae) Quench the Quorum Sensing of Pseudomonas aeruginosa PAO1." Journal of Intercultural Ethnopharmacology 3.4 (2014), 138-143. Print. doi:10.5455/jice.20140710031312



APA (American Psychological Association) Style

Okusa, P. N., Rasamiravaka, T., Vandeputte, O., Stévigny, C., Jaziri, M. E. & Duez, P. (2014) Extracts of Cordia gilletii De Wild (Boraginaceae) Quench the Quorum Sensing of Pseudomonas aeruginosa PAO1. Journal of Intercultural Ethnopharmacology, 3 (4), 138-143. doi:10.5455/jice.20140710031312





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