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Guava leaves polyphenolics-rich extract inhibits vital enzymes implicated in gout and hypertension in vitro

Emmanuel Anyachukwu Irondi, Samson Olalekan Agboola, Ganiyu Oboh, Aline Augusti Boligon, Margareth Linde Athayde, Francis O. Shode.

Cited by (8)

Abstract
Background/Aim: Elevated uric acid level, an index of gout resulting from the over-activity of xanthine oxidase (XO), increases the risk of developing hypertension. However, research has shown that plant-derived inhibitors of XO and angiotensin 1-converting enzyme (ACE), two enzymes implicated in gout and hypertension, respectively, can prevent or ameliorate both diseases, without noticeable side effects. Hence, this study characterized the polyphenolics composition of guava leaves extract and evaluated its inhibitory effect on XO and ACE in vitro. Materials and Methods: The polyphenolics (flavonoids and phenolic acids) were characterized using high-performance liquid chromatography (HPLC) coupled with diode array detection (DAD). The XO, ACE, and Fe2+-induced lipid peroxidation inhibitory activities, and free radicals (2,2-diphenylpicrylhydrazyl [DPPH]* and 2,2´-azino-bis-3-ethylbenzthiazoline-6-sulphonic [ABTS]*+) scavenging activities of the extract were determined using spectrophotometric methods. Results: Flavonoids were present in the extract in the order of quercetin > kaempferol > catechin > quercitrin > rutin > luteolin > epicatechin; while phenolic acids were in the order of caffeic acid > chlorogenic acid > gallic acids. The extract effectively inhibited XO, ACE and Fe2+-induced lipid peroxidation in a dose-dependent manner; having half-maximal inhibitory concentrations (IC
50) of 38.24 ± 2.32 μg/mL, 21.06 ± 2.04 μg/mL and 27.52 ± 1.72 μg/mL against XO, ACE and Fe2+-induced lipid peroxidation, respectively. The extract also strongly scavenged DPPH* and ABTS*+. Conclusion: Guava leaves extract could serve as functional food for managing gout and hypertension and attenuating the oxidative stress associated with both diseases.

Key words: Angiotensin I-converting enzyme, Fe2+-induced lipid peroxidation, oxidative stress, xanthine oxidase


 
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How to Cite this Article
Pubmed Style

Irondi EA, Agboola SO, Oboh , Boligon AA, Athayde ML, Shode O. Guava leaves polyphenolics-rich extract inhibits vital enzymes implicated in gout and hypertension in vitro. J Intercult Ethnopharmacol. 2016; 5(2): 122-130. doi:10.5455/jice.20160321115402



Web Style

Irondi EA, Agboola SO, Oboh , Boligon AA, Athayde ML, Shode O. Guava leaves polyphenolics-rich extract inhibits vital enzymes implicated in gout and hypertension in vitro. www.scopemed.org/?mno=217470 [Access: November 18, 2017]. doi:10.5455/jice.20160321115402



AMA (American Medical Association) Style

Irondi EA, Agboola SO, Oboh , Boligon AA, Athayde ML, Shode O. Guava leaves polyphenolics-rich extract inhibits vital enzymes implicated in gout and hypertension in vitro. J Intercult Ethnopharmacol. 2016; 5(2): 122-130. doi:10.5455/jice.20160321115402



Vancouver/ICMJE Style

Irondi EA, Agboola SO, Oboh , Boligon AA, Athayde ML, Shode O. Guava leaves polyphenolics-rich extract inhibits vital enzymes implicated in gout and hypertension in vitro. J Intercult Ethnopharmacol. (2016), [cited November 18, 2017]; 5(2): 122-130. doi:10.5455/jice.20160321115402



Harvard Style

Irondi, E. A., Agboola, S. O., Oboh, ., Boligon, A. A., Athayde, M. L. & Shode, . O. (2016) Guava leaves polyphenolics-rich extract inhibits vital enzymes implicated in gout and hypertension in vitro. J Intercult Ethnopharmacol, 5 (2), 122-130. doi:10.5455/jice.20160321115402



Turabian Style

Irondi, Emmanuel Anyachukwu, Samson Olalekan Agboola, Ganiyu Oboh, Aline Augusti Boligon, Margareth Linde Athayde, and Francis O. Shode. 2016. Guava leaves polyphenolics-rich extract inhibits vital enzymes implicated in gout and hypertension in vitro. Journal of Intercultural Ethnopharmacology, 5 (2), 122-130. doi:10.5455/jice.20160321115402



Chicago Style

Irondi, Emmanuel Anyachukwu, Samson Olalekan Agboola, Ganiyu Oboh, Aline Augusti Boligon, Margareth Linde Athayde, and Francis O. Shode. "Guava leaves polyphenolics-rich extract inhibits vital enzymes implicated in gout and hypertension in vitro." Journal of Intercultural Ethnopharmacology 5 (2016), 122-130. doi:10.5455/jice.20160321115402



MLA (The Modern Language Association) Style

Irondi, Emmanuel Anyachukwu, Samson Olalekan Agboola, Ganiyu Oboh, Aline Augusti Boligon, Margareth Linde Athayde, and Francis O. Shode. "Guava leaves polyphenolics-rich extract inhibits vital enzymes implicated in gout and hypertension in vitro." Journal of Intercultural Ethnopharmacology 5.2 (2016), 122-130. Print. doi:10.5455/jice.20160321115402



APA (American Psychological Association) Style

Irondi, E. A., Agboola, S. O., Oboh, ., Boligon, A. A., Athayde, M. L. & Shode, . O. (2016) Guava leaves polyphenolics-rich extract inhibits vital enzymes implicated in gout and hypertension in vitro. Journal of Intercultural Ethnopharmacology, 5 (2), 122-130. doi:10.5455/jice.20160321115402





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