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Detection of potential AcrAB-TolC multidrug efflux pump inhibitor in calyces extract of Hibiscus sabdariffa

Nehaya Al-Karablieh, Luay Abu-Qatouseh, Talal Aburjai.

Abstract
Aim: The aim of this study is to investigate occurrence of potential efflux pump inhibitor (EPI) against AcrAB-TolC efflux pump in the methanol extract of H. sabdariffa. Materials and Methods: Calyces of H. sabdariffa were purchased from the local market in April 2014, methanol extract of H. sabdariffa was subjected to agar plate diffusion against Escherichia coli TG1 and its ∆acrB-∆tolC and thin layer chromatography (TLC) bioassay. The corresponding EPI fraction was eluted by methanol. The synergistic effect of antimicrobials and EPI fraction was measured by minimum inhibitory concentration (MIC) determination for E. coli and Erwinia amylovora strains, and the ability of EPI fraction to enhance EtBr accumulation was conducted. Results: E. coli TG1 was more sensitive to the methanol extracts of H. sabdariffa than E. coli ∆acrB-∆tolC, and inhibition zone corresponding to flavones on TLC bioassay plate has been formed which might be related to the fraction of potential EPI. The MIC values revealed that EPI fraction enhanced the activity of the used antimicrobials by 4 to 8 folds in E. coli TG1 and by 4 to 10 folds in E. amylovora 1189. Addition of EPI fraction in a dose-dependent manner increased the intercellular accumulation of Ethidium Bromide (EtBr) in the wild type stains of E. coli TG1 and E. amylovora 1189. Conclusion: EPI fraction behaves like a multidrug efflux pump inhibitor and further investigation should be conducted for determination of the chemical structure of EPI fraction.

Key words: AcrAB-TolC, Efflux pump inhibitor, Hibiscus sabdariffa


 
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Pubmed Style

Al-Karablieh N, Abu-Qatouseh L, Aburjai T. Detection of potential AcrAB-TolC multidrug efflux pump inhibitor in calyces extract of Hibiscus sabdariffa. J Intercult Ethnopharmacol. 2017; 6(4): 357-363. doi:10.5455/jice.20170917062504


Web Style

Al-Karablieh N, Abu-Qatouseh L, Aburjai T. Detection of potential AcrAB-TolC multidrug efflux pump inhibitor in calyces extract of Hibiscus sabdariffa. http://www.jicep.com/?mno=266799 [Access: December 15, 2017]. doi:10.5455/jice.20170917062504


AMA (American Medical Association) Style

Al-Karablieh N, Abu-Qatouseh L, Aburjai T. Detection of potential AcrAB-TolC multidrug efflux pump inhibitor in calyces extract of Hibiscus sabdariffa. J Intercult Ethnopharmacol. 2017; 6(4): 357-363. doi:10.5455/jice.20170917062504



Vancouver/ICMJE Style

Al-Karablieh N, Abu-Qatouseh L, Aburjai T. Detection of potential AcrAB-TolC multidrug efflux pump inhibitor in calyces extract of Hibiscus sabdariffa. J Intercult Ethnopharmacol. (2017), [cited December 15, 2017]; 6(4): 357-363. doi:10.5455/jice.20170917062504



Harvard Style

Al-Karablieh, N., Abu-Qatouseh, L. & Aburjai, T. (2017) Detection of potential AcrAB-TolC multidrug efflux pump inhibitor in calyces extract of Hibiscus sabdariffa. J Intercult Ethnopharmacol, 6 (4), 357-363. doi:10.5455/jice.20170917062504



Turabian Style

Al-Karablieh, Nehaya, Luay Abu-Qatouseh, and Talal Aburjai. 2017. Detection of potential AcrAB-TolC multidrug efflux pump inhibitor in calyces extract of Hibiscus sabdariffa. Journal of Intercultural Ethnopharmacology, 6 (4), 357-363. doi:10.5455/jice.20170917062504



Chicago Style

Al-Karablieh, Nehaya, Luay Abu-Qatouseh, and Talal Aburjai. "Detection of potential AcrAB-TolC multidrug efflux pump inhibitor in calyces extract of Hibiscus sabdariffa." Journal of Intercultural Ethnopharmacology 6 (2017), 357-363. doi:10.5455/jice.20170917062504



MLA (The Modern Language Association) Style

Al-Karablieh, Nehaya, Luay Abu-Qatouseh, and Talal Aburjai. "Detection of potential AcrAB-TolC multidrug efflux pump inhibitor in calyces extract of Hibiscus sabdariffa." Journal of Intercultural Ethnopharmacology 6.4 (2017), 357-363. Print. doi:10.5455/jice.20170917062504



APA (American Psychological Association) Style

Al-Karablieh, N., Abu-Qatouseh, L. & Aburjai, T. (2017) Detection of potential AcrAB-TolC multidrug efflux pump inhibitor in calyces extract of Hibiscus sabdariffa. Journal of Intercultural Ethnopharmacology, 6 (4), 357-363. doi:10.5455/jice.20170917062504





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