E-ISSN 2577-5669
 

Research Article 


Isolation, Identification, Characterization and Antimicrobial Study of Probiotic from Sauerkraut

WADULKAR R.D, BHUSNURE O.G, MORE R.A.

Abstract
Background and Objectives: Long term use of Antibiotics, sedentary lifestyle, and change in the diet pattern is responsible for dysbiosis of microbiota. Dysbiosis of microbiota is the etiological factors of chronic diseases. Probiotics are live microorganisms, which show beneficial health effects on hosts once consumed in sufficient amounts may be the prophylaxis approach for chronic disease. LAB group can be isolated and characterized from traditional dairy sources and fermented product. This study aimed at isolating, identifying, and in vitro characterizing (morphology, catalase, oxidase test 16 S rRNA Sequencing and Phylogenetic Analysis) LAB strains from traditional Sauerkraut.
Materials and Methods: Isolated strains were identified by Gram staining, catalase assay, and molecular identification methods; 16S rDNA gene sequencing, phylogeny study, antipathogenic study and antibiotic susceptibility Assay.
Results: The experiments revealed that Pediococcus pentosaceus DSM 20336 (T) strains, which were isolated sauerkraut shows a desirable tolerance to low pH and high bile salts, favourable anti-pathogen activity, and acceptable antibiotic susceptibility; hence, they could be considered as novel probiotic candidates for preventing and treating the chronic disease

Key words: Dysbiosis, Microbiota, Sauerkraut, Pediococcus pentosanes, probiotic


 
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How to Cite this Article
Pubmed Style

R.D W, O.G B, R.A M. Isolation, Identification, Characterization and Antimicrobial Study of Probiotic from Sauerkraut. J Complement Med Res. 2021; 12(2): 253-261. doi:10.5455/jcmr.2021.12.02.29


Web Style

R.D W, O.G B, R.A M. Isolation, Identification, Characterization and Antimicrobial Study of Probiotic from Sauerkraut. https://www.jocmr.com/?mno=15488 [Access: June 24, 2022]. doi:10.5455/jcmr.2021.12.02.29


AMA (American Medical Association) Style

R.D W, O.G B, R.A M. Isolation, Identification, Characterization and Antimicrobial Study of Probiotic from Sauerkraut. J Complement Med Res. 2021; 12(2): 253-261. doi:10.5455/jcmr.2021.12.02.29



Vancouver/ICMJE Style

R.D W, O.G B, R.A M. Isolation, Identification, Characterization and Antimicrobial Study of Probiotic from Sauerkraut. J Complement Med Res. (2021), [cited June 24, 2022]; 12(2): 253-261. doi:10.5455/jcmr.2021.12.02.29



Harvard Style

R.D, W., O.G, . B. & R.A, . M. (2021) Isolation, Identification, Characterization and Antimicrobial Study of Probiotic from Sauerkraut. J Complement Med Res, 12 (2), 253-261. doi:10.5455/jcmr.2021.12.02.29



Turabian Style

R.D, WADULKAR, BHUSNURE O.G, and MORE R.A. 2021. Isolation, Identification, Characterization and Antimicrobial Study of Probiotic from Sauerkraut. Journal of Complementary Medicine Research, 12 (2), 253-261. doi:10.5455/jcmr.2021.12.02.29



Chicago Style

R.D, WADULKAR, BHUSNURE O.G, and MORE R.A. "Isolation, Identification, Characterization and Antimicrobial Study of Probiotic from Sauerkraut." Journal of Complementary Medicine Research 12 (2021), 253-261. doi:10.5455/jcmr.2021.12.02.29



MLA (The Modern Language Association) Style

R.D, WADULKAR, BHUSNURE O.G, and MORE R.A. "Isolation, Identification, Characterization and Antimicrobial Study of Probiotic from Sauerkraut." Journal of Complementary Medicine Research 12.2 (2021), 253-261. Print. doi:10.5455/jcmr.2021.12.02.29



APA (American Psychological Association) Style

R.D, W., O.G, . B. & R.A, . M. (2021) Isolation, Identification, Characterization and Antimicrobial Study of Probiotic from Sauerkraut. Journal of Complementary Medicine Research, 12 (2), 253-261. doi:10.5455/jcmr.2021.12.02.29